CHEM 2121 Lecture Notes - Bacteriophage, Wireless Lan, Retardation Factor
Document Summary
Removing some species from 1 phase to another. At equilibrium, [a]aq = q(mol a)/vaq where q = fraction remaining in aqueous phase. [a]org = (1 - q)(mol a)/vorg; plug back into k eqn and solve for q. Fraction remaining depends on v aq and partition coefficientsthe larger the k (partition coefficient), the smaller the fraction remaining. If multiple identical extractionsraise q to the n (# extractions. -> q = 20. 8 % remaining in aq phase. -> q = 5. 9% better to do many smaller extractions than 1 large extraction ex: cleaning glassware: better to do multiple small rinses than 1 large rinse. Assuming that there"s separation of phases ( 1 mobile phase and 1 stationary phase) Mobile (eluent) phase: solvent moving through column (gas or liquid) -> 2 types of columns in stationary phase. Packed column: pack stationary phase particles in column. Open tubular column: stationary phase bound to inner walls of tube.
