1 - M Q I F V K T L T G K T I T L E V E P S D T I E N - 25
26 - V K A K I Q D K E G I P P D Q Q R L I F A G K Q L - 50
51 - E D G R T L S D Y N I Q K E S T L H L V L R L R G â 75
76 - G
1. Estimate the pI for this protein. Explain your reasoning and show your work.
2. What form of ion-exchange chromatography would you use to purify this protein at a pH of 5.9? Explain your reasoning.
