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BCMB20005- Final Exam Guide - Comprehensive Notes for the exam ( 41 pages long!)

542 views41 pages
School
University of Melbourne
Department
Biochemistry and Molecular Biology
Course
BCMB20005
Professor
Amber Willems

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Document Summary

Pipetting techniques, as pipettes are used in almost all practicals. Method of spectrophotometry and gel electrophoresis: use in molecular biology and biochemistry. Common tools that are used in this field: molecular biology and biochemistry. How does it work: plunger provides suction to pull liquid into the tip. Pushing down the piston to the first pressure point and releasing it slowly creates a partial vacuum. This allows the liquid to be sucked up. When pushed down to the second pressure point, the liquid is released: adjustment button allows the maximum volume needed to be up taken. Accuracy: the degree of (cid:862)agree(cid:373)e(cid:374)t of a set of (cid:373)easure(cid:373)e(cid:374)ts to the true (cid:448)alue(cid:863) Precision: the degree to which repeated measurements agree closely with each other (reproducible). What to do: pipette should always be vertical, maintain a constant rhythm, check all the liquid has been delivered volume errors can result in poor or false data.

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