DEV2011 Study Guide - Final Guide: Confocal Microscopy, Endoplasmic Reticulum, Primary And Secondary Antibodies
14 Apr 2020
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And the light patches are known as euchromatin. Chromatin is bound to basic proteins known as histones. The dense patches of the nucleus are called heterochromatin. Name the structure that divides the nucleus from the cytoplasm nuclear membrane. Plasma membrane separates cytoplasm from surrounding environment. Smooth endoplasmic reticulum generation and metabolism of lipids and steroids, Select one: confocal microscope, light microscope, electron microscope. Use a fluorescent antibody to bind the protein, and use a confocal microscope to visualise. Use a primary antibody to bind the protein, then a fluorescent secondary antibody, and visualise with a confocal microscope. Use a primary antibody, then an enzyme-conjugated secondary antibody, then apply the enzyme substrate and visualise with a light microscope. Confocal microscopy following detection with a protein-specific antibody is perfect for detecting transcription factors and their subcellular localization. The major function of transcription factors is to bind to promoters/regulatory regions of specific target genes.
