BIOL2200 Study Guide - Final Guide: Homeostasis, Phagocytosis, Digestion

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15 Sep 2017
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The airy disk: various points that pass-through objective which make up the image seen = caused by diffraction/scattering of light when it passes through specimen; if too much overlap of airy disks = blur of signals. Resolution: limit up to which two small objects are still seen as separate entities; measure of resolving power = do. Prokaryotes = simple structure; eukaryotes = many membrane limited organelles (em good for this) Visualises relative distributions between two proteins; spatial relationship and overlap of sig(cid:374)als (cid:272)a(cid:374) (cid:271)e see(cid:374) (cid:894)sig(cid:374)als (cid:272)a(cid:374)(cid:859)t (cid:272)olo(cid:272)alise(cid:895) Dyes used absorb light at one set wavelength and emit light at another set of wavelengths. Max. absorbance = most energetic state = excitation = best absorbance wavelength. Stokes shift = difference between peak emission and peak absorption wavelengths; allows for analysis. -> for this reason special filters are used to restrict bandwidths and differentiate between the two; also, allow for multiple dyes to be used simultaneously.

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