BIOL10004 Study Guide - Final Guide: Fetal Alcohol Spectrum Disorder, Dna Ligase, Okazaki Fragments
8 Sep 2018
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Document Summary
A short rna sequence called the primer, which is produced by primase, is added to the 3(cid:859)oh free end to initiate replication. Dna polymerase iii starts to synthesize nucleotides onto new strands following complementary base paring rule as the helicase further separating the strands. For leading strand, only one primer is needed to initiate replication because it synthesizes in 5(cid:859) to 3(cid:859) direction. Topoisomerase (gyrase) is used at the end of double strand to release tension after being cut by helicase. However, the other strand, termed lagging strand, needs multiple primers because primers does not code in 3(cid:859) to 5(cid:859) direction. Therefore, dna polymerase iii adds nucleotides to lagging strand as a series of segment, each with a primer to initiate. Dna polymerase i comes and replace primers with dna sequence together with checking the correctness of previous replication. In broad terms the similarities and difference between replication in a prokaryote and eukaryote.
