91344 Final: Medical Diagnostics Biochemistry
Document Summary
Introductory principles: physical safety, chemical safety, biological safety. Laboratory needs to contain: a safety officer, first aid officer and first aid kit, fire extinguisher and fire blankets, corridors and fire exits are all free, dangerous chemicals should be stored safely, sharps in the sharp box. Chemical safety: eye wash station, fume hoods, negative pressure rooms and showers for acid spills, be aware of flammable, explosive, acidic and flash points of chemicals, explosive compounds usually contain instable o-o, n-n or. Hepatotoxins: usually heavily chlorinated (cl) carbon compounds (eg: tetrachloride ccl4) Carcinogens: o-tolidine (aromatic amines) previously used a redox indicator, naphthalene (polycyclic aromatics) and methyl nitrosamine (nitroso compounds) Primarily concerned with infectious bacteria and viruses such as hiv- Why: proteins can cause spectral interference, react with reagents to cause excessive foaming or precipitates, turbidity and foaming cause issues. Chloroform is another option which makes the proteins act like a surfactant.
