300936 Study Guide - Final Guide: Endoplasmic Reticulum, Glycosylation, Golgi Apparatus
Document Summary
Recall the major amino acid targets for glycosylation of proteins. Outline glycosylation of proteins including the progress through different cellular compartments where the glycosylation occurs. Compare and contrast n -glycosylation with o -glycosylation. Occurs on the amide (n) group of the amino acid asparagine, located in the membrane. N-linked glycosylation: is the attachment of an oligosaccharide on the nitrogen. O-linked glycosylation: is the attachment of an oligosaccharide on the oxygen. List the reasons for protein glycosylation: promotes folding and stability, modifies cells, protein function. Explain the basic principles of international enzyme classification. Enzymes: are biological catalysts that speed up the rate of reaction. Are substrate specific, due to the precise interaction of the substrate with the enzyme as a result of the 3d structure of the enzyme protein. Are flexible and are denatured when too flexible. Explain that enzyme catalysis does not affect the equilibrium of a reaction but increases the speed of a reaction.
