300936 Study Guide - Final Guide: Conformational Ensembles, Anabolism, Catabolism
Document Summary
Describe the properties of allosteric enzymes and their roles in regulation of biochemical pathways; Allosteric enzymes are enzymes that change their conformational ensemble upon binding to an effector, which results in an apparent change in binding affinity at a different ligand binding site. Properties: one of the groups experiences only a change in affinity for the pri(cid:374)(cid:272)ipal su(cid:271)strate, while keepi(cid:374)g the (cid:373)a(cid:454)i(cid:373)u(cid:373) rate fairl(cid:455) (cid:272)o(cid:374)sta(cid:374)t ( k t(cid:455)pe enzymes). The second group also demonstrates significant changes in affinity for the main substrate, and in addition has large changes in the maximum rate ( v typeenzymes). The greatest changes in v max are for enzymes that act as regulatory switches. Function: catalyse reactions in metabolic pathways, control the rate of these pathways. Regulation by allosteric enzymes involves the binding of signalling molecules to specific regulatory sites, and this binding induces conformational changes which results in alterations in catalytic activity. Metabolism: is the sum of catabolism and anabolism.
