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BIOL 200 Study Guide - Kozak Consensus Sequence, Palindromic Sequence, Tata Box


Department
Biology (Sci)
Course Code
BIOL 200
Professor
Richard Roy

Page:
of 2
Lecture16- Molecular Genetics
1
Cloning (physically splitting/replacing nucleus/fragment into plasmid)
Palindromic sequence
Cohesive overhang ends
Vector-specialized plasmid
Polylinker
Labile mRNA
Transient transfection
Stable transfection (transformation)
Fusion protein
Qualitative analysis (size, nucleotide composition, conformation, structure)
Quantitative analysis (level of product… ie tumor markers)
Molecular probes (fluorescent, radioactive)
Degenerate pool
Alkaline solution
Capillary action
Blot
Hybridazation
Non-specific signal
Autoradiography
Lecture17- Eukaryotic Transcription
Compartmentalized transctription (plastid, mitochondria, nucleus)
Initiator sequence for transcription
Promoter/initiator sequences (TATA box)
Preinitiation complex
Limiting step in transcription
Lecture18- Eukaryotic Transcription 2
Recognition helix of transcription factors
Homeotic transformation
Homeodomain protein
Coactivators
Lecture19- RNA Processing 1
RNA-RNA pairing critical for U1 function
Contiguous sequence for spiced exons
Lecture20- RNA Processing 2
Maturation of nascent transcript
Capping
Splicing
Polyadenylating
RNA editing
Lecture21- Nucleo-Cytopl Transport
Nuclear import of NLS-containing cargo
Nuclear export of NES-containing cargo
mRNA export
mRNP remodelling
Lecture22- Protein Synthesis 1
Pre-tRNA processing:
Cleave 5’
Remove an intron
Add CCA to 3’
Modify internal bases
Pre-rRNA transcription unit repetive units in nucleolus
Prokaryote ribosome:
50S large subunit-5S+23S +31proteins
30S small subunit-16S +21 proteins
Eukaryotic ribosome:
60S large subunit-5S +5.8S + 28S+ 50proteins
40S small subunit-18S+ 33 proteins
Pre-initiation complex (40S + 3 + 1A + 2-GTP-Met)
Initiation complex (40S + 3 + 1A + 2-GTP-Met +4)
Shine-Dalgarno box
Pairs with 16S
Kozak sequence (ACC-AUG-G)
Lecture23- Protein Synthesis 2
tRNA processing
High energy ester bond
Reinitiation/initiation complex
Elongation
Translation termination
Reinitiation
Lecture24- Protein Folding and Quality
Control
Decapping pathway
Deadenylation-dependant pathway
Endonucleolytic pathway
Pionieering round of translation
Nonsense mutation
Nonsense-mediated decay
Chaperones
Chaperonins
prions
Lecture25- Transl and Post-Transl
regulation of gene expression
Unfolded protein response (UPR)
Phosphorylation
Ubiquitination
Degradation
Lecture26- Proteins: Structure, Function,
Strategies
X-ray diffraction
Scatter pattern
Electon density
Electron density map/diagram
Protein topology
Ligand
Allosteric switch
Dissociation constant Kd
4 Calcium + Calmodulin
GTP-binding proteins
GAP
GEF
Protein kinase
Protein phosphatase
Protein conformation
Lecture27- Chromatin Modifications
Mating type
Transcriptional repression
Histone code
Heterochromatin
Euchromatin
Dosage compensation
Calico cats
Epigenetics
Epigenetic traits
Lecture28- New Roles for mRNA
Chromosomal silencing
RNA world
Systemic effect
Lecture29-
Functional genomics
Disruption construct/Gene targeting/Targeting construct/vector
Trypsin proteolisis
Peptide mass fingerprint
modularity
Bait domain
Fish domain (prey)
Selectable marker/ reporter
Prototrophic selection
Having the nutrient requirements of the wild type
Interactome
Lecture30-
Bait domain
Fish domain (prey)
Reporter gene
Forward genetics
Function-> gene
Reverse genetics
Gene-> funccion
Gene targeting
Dominant selective marker
Flaking regions
Pluripotent cell
Embryonic stem cells (ES cells)
Totipotent cells
Blastocyst
Chimera
Transgenic mice