BIOL 1202 Study Guide - Midterm Guide: Scanning Electron Microscope, Gram Staining, Field Of View
22 Oct 2016
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Microscopy 1: an introduction to the use of microscopes. Identify features of a compound and dissecting microscope and explain their functions. Define and explain magnification, working distance, illumination, revolving power and focus. Understand how magnification affects resolution, and how to manipulate light. Describe the use of compound and dissecting microscopes, and key terminology (eg. resolution, magnification, parts of microscope) Define and explain: fov; parfocal; real image (right side up); virtual image (inverted) Understand the differences between light and electron microscopes (and between transmission and scanning electron microscope; tem + sem) Proper slide preparation- wet mounts and simple staining. The blue light condenser has more clarity as it has a shorter wavelength to penetrate better. This is different because the compound light has a convex. Why does it look different than without a microscope? lens, which inverts the image (diverges light). The compound microscope has a concave lens in the objective lenses and convex in ocular.
