CMMB 343 Study Guide - Quiz Guide: Resazurin, Anaerobic Respiration, Oxidase Test
5 May 2019
School
Course
Professor
Document Summary
Helps identify if an organism is capable of producing catalase. Inoculate organism into a tube of molten tryptose agar. As a result of fermentation, gas production will cause the tryptose agar to crack. Uses lytic cycle, instead of lysogenic cycle. > pyruvic acid (reduced entirely) -> lactic acid. Single gene knockouts are used to study the effects of losing the function of specific genes on an organism. By counting the plaques formed in the secondary host culture. Anaerobic respiration or fermentation when oxygen is not present. Facultative organisms either respire or ferment glucose depending on the presence or absence of oxygen. Transformed cells will now have the antibiotic resistance gene included in the plasmid, allowing transformants to grow in media containing the selected antibiotic. Contains a tn5 (kanr) insertion in its genome. Mutant with a stop codon in the middle of one of the genes necessary for replication. Only replicate in hosts that contain a suppressor mutation.
