BIOC 4580 Final: BIOC 4580 Class 28 notes
21 Mar 2017
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Bioc 4580 class 28: cell surface carbohydrates i. Carbohydrates attach to membrane proteins & this moiety ends up extracellularly indeed, it forms a glycocalyx coat. The erythrocyte glycocalyx is due to large carbohydrate chains on glycophorin (determining blood group types) and small ones on band 3. Erythrocyte glycosphingolipids also contribute to blood group types. Most other cell types have an extracellular matrix derived from glycosaminoglycans, proteoglycans, fibronectins, & integrins. Lectins (carbohydrate-binding proteins, with antibody-like specificities) & glycosidases (digestive enzymes) are useful tools in glycomics, followed by mass spectrometry and/or nmr spectroscopy. Erythrocyte glycocalyx is formed by glycophorin, band 3, & Oligosaccharides form a 140(ish) nm thick coat for protection & surface antigenicity, and stability. O-linked sugars and - amino acid sequence make up the mn blood group antigens & are genetically determined. Mn blood group system is based on glycophorins. A pair of blood group antigens governed by genes that segregate independently of the abo locus.
