MBG 2040 Study Guide - Final Guide: Operon, Lac Repressor, Dysgenics

• School: University of Guelph
• Department: Molecular Biology and Genetics
• Course: MBG 2040
• Professor: James Uniacke

Document Summary

Discovery of reverse transcriptase in retroviruses and retro-like viruses defied the central dogma proposed by crick in. 1958 that [dna transcribed to rna which was translated to polypeptide (amino acids)]. Dna goes to rna vice versa (reverse transcriptase) but only rna can be translated to protein. Anti-parallel strands for 5" to 3" polarity. B-form dna (right handed double helix); most common form of cells in living organisms. Watson and crick"s dna replication showed a semi-conservative model of replication. Meeselson and stahl eliminated the other possibilities to prove that semi-conservative was the true copying mechanism. Unlike in eukaryotes, e. coli has only one origin of replication (oric). Oric has 13 at rich mer repeats and four. There are 4 binding sites for the initiator protein dnaa. Dna replication is bi-directional from origin of replication. Requires primer with free oh group at 3" end. Needs template dna strand to specify sequence of new dna strand.