MCB 2050 Study Guide - Midterm Guide: Southern Blot, Northern Blot, Dna Microarray

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Published on 19 Apr 2013
School
University of Guelph
Department
Molecular and Cellular Biology
Course
MCB 2050
Professor
MCB 2050 Midterm exam outline
Winter 2013 (RAY LU)
Multiple choice: 19 marks
Short answer: 18 marks
Southern blotting can be used to determine if a genomic DNA clone has a particular STS
Ori is a common feature of all cloning vectors
DNA palindrome
5’ AATGCCGGCATT 3’
3’ TTACGGCCGTAA 5’
DNA ligase joins the ends of two DNA fragments
DNA polymerase is required in sanger’s DNA sequencing reaction
cDNA is produced with reverse transcriptase
Contigs are overlapping genomic DNA clones
HAT complex is involved in chromosome remodeling
DNA microarray technology for transcription profiling Hybridize fluorescently labeled
cDNA sample to DNA probes on the array
Luman is activated by proteolytic cleavage and regulated by post-translational regulation
miRNA’s usually result in the repression of translation of the target mRNA
according to the human Genome project the percent of human genome containing exons
that code for proteins is 1%
Transcription of a gene, splicing of an RNA molecule, migration of an mRNA molecule
into the cytoplasm, polypeptide synthesis, degradation of an mRNA molecule
Zn finger domains are DNA binding domains
SNP’s on a chromosome segment are inherited together and define genetic unit is called
haplotype
Chimeras are animals that have two types of cells that have sontributed to the formation
of adult tissue
Gene imprinting primarily caused by DNA methylation
Know the difference between a promoter and enhancer and things like orientation
dependent/ independent, location in the gene, its effect on expression
Two techniques to test for expression of a gene are:
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Document Summary

Southern blotting can be used to determine if a genomic dna clone has a particular sts. Ori is a common feature of all cloning vectors. Dna ligase joins the ends of two dna fragments. Dna polymerase is required in sanger"s dna sequencing reaction. Hat complex is involved in chromosome remodeling. Dna microarray technology for transcription profiling hybridize fluorescently labeled cdna sample to dna probes on the array. Luman is activated by proteolytic cleavage and regulated by post-translational regulation. Mirna"s usually result in the repression of translation of the target mrna. According to the human genome project the percent of human genome containing exons that code for proteins is 1% Transcription of a gene, splicing of an rna molecule, migration of an mrna molecule into the cytoplasm, polypeptide synthesis, degradation of an mrna molecule. Zn finger domains are dna binding domains. Snp"s on a chromosome segment are inherited together and define genetic unit is called haplotype.

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