MCB 2050 Final: MCB Exam Review

• School: University of Guelph
• Department: Molecular and Cellular Biology
• Course: MCB 2050
• Professor: John Vessey Richard Mosser

Document Summary

Bright-field microscopy components: light source, condenser lens, stage, objective and ocular lens light diffracted by specimen and undiffracted light focused by objective lens. More sensitive to low light intensities allowing for living cells to be viewed w/o damage. Principle of deconvolution: improves contrast and resolution of microscopic images. Magnification= objective lens x digital lens resolution: minimum distance that separate 2 points while still distinguishable as separate point (depends on wavelength and numerical aperature) Fluorescence microscopy: uses fluorophores (molecules that can re-emit light following excitation) Confocal laser scanning microscopy (clsm) one or more lasers allow only certain wavelengths of light to strike and. Excite" the living specimen allows dynamic processes to be viewed. Z-section/ optical sectioning: clsm focused on single layer within a specimen. Beam of electrons instead of light generate highly magnified views of internal structures shows density by diffraction (diffracted areas=electron dense= black, non- diffracted=white)