MCIM 326 Study Guide - Final Guide: Proline, Penicillin, Wild Type
16 Dec 2015
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Questions 1-28 require some analysis or a short answer. Questions 33-71 (not consecutive) are t/f: does the symbol lac+ refer to the genotype or phenotype of a bacterium. Ans: an absolute defective mutant has a mutant phenotype in all conditions; a conditional mutant has a mutant phenotype only in certain conditions: insert the terms pen-r and pen-r into the following sentence. Ans: a pen-r mutant contains a pen-r mutation. Ans: the phenotype is leu+, because the + allele is dominant. It is not temperature sensitive: the frequency of producing a mutant arb- is 2x10-6 per generation and mutant snd- is 8x10-5. Ans: (2x10-6) (8x10-5) = 1. 6x10-10: rank the following mutations in order of reversion frequency: deletion, point mutation, double mutation. If it was, vase would be temperature sensitive: a mutation prevents the synthesis of substance z and results in the accumulation of large amounts of substance r, which is normally present in only very small amounts.
