BIOL 241- Midterm Exam Guide - Comprehensive Notes for the exam ( 13 pages long!)
11 Oct 2017
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These infections involve the liberation of endotoxins. Infections involve the liberation of endotoxins: aeruginosa, freundii, subtillis, europea, cereus, fluorescens, vulgaris, vinelandii. Nessler"s reagent: + (brown) ph: 7 (green) Nessler"s reagent: + (brown) ph: 7 (green) urea: no ammonia (yellow) Nessler"s reagent: ++ ph: 7 green urea: ammonia pink. No water insoluble pigment but a water soluble florescent pigment is produced: chroococcum. No water soluble pigment so no fluorescence. Water insoluble brown pigment which in some strains later becomes black: beijerinckii. Cells can be singly or in pairs: paspali. Rhizobium cells cells can form cysts, turn yellow, cinnamon with water insoluble pigment. Large, ovoid, pleomorphic rod coccoid cells to elongated filaments. Water-soluble florescent pigment is produced which fluoresces green under uv light no water insoluble pigment is produced. Forms a membrane which invaginates inward extending into secondary roots. Oxidizes sulfur and thiosulfates to sulfuric acid aerobically. Great resistance to acid: desulfuricans ph 1-3. Therefore b. subtilis is not a coliform bacteria.
