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The Comparative Report on Different Techniques of Staining Bacterial Cell.docx

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BIOL 354
Julia Roberts

The Comparative Report on Different Techniques of Staining Bacterial CellSunny Kang20369516Teaching Assistants Anshula S Jeff KrukBiol 240LndthMay 22 2013June 5 2013Introduction The purpose of this experiment was to compare and contrast the different techniques and observe the benefits as well as the drawbacks of each method The gram stain method was used to differentiate between two groups of bacteria the Grampositive and the Gramnegative Bruckner 2012 The Grampositive and the Gramnegative stain differentiate in many ways but for the gram stain method the main difference to observe is the cell wall Grampositive bacteria have a thick peptidoglycan cell wall while Gramnegative bacteria have a thin peptidoglycan cell wall Rollins D Joseph S 2000 The Bacteria used for this method were Staphylococcus Epidermidis and Escherichia Coli which are Grampositive and Gramnegative respectfully redOrbit 2013 The first step for this method was to use the primary stain crystal violet then the Grams iodine solution which is added to make the stain insoluble to water fixing the stain to the cell Bruckner 2012 Then the culture was decolorized with ethyl alcohol which dehydrates the cell wall so the stain will be trapped in a thick peptidoglycan layer that has tightened and alternatively the outer membrane will degrade in a thin peptidoglycan layer Bruckner 2012 Finally the counterstain safranin is used to stain the subsequent decolorized Gramnegative cell which will appear red and the nondecolorized Grampositive cell will appear purple This method is simple fast and relatively effective in differentiating Gram cells The disadvantages are that this technique requires fresh cultures because the older cultures will stain both as Grampositive and Gramnegative and during the decolorization Grampositive cells can degrade if the agent is left on for too long Bruckner 2012
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