BIOL432 Study Guide - Final Guide: Oxaloacetic Acid
14 Apr 2015
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Fed batch fermentation: substrate added periodically during fermentation, once or twice but no more (cid:224) prolong log and stationary phase, problem: proteolytic enzymes are produced in stationary phase, used for animal cell culture. 17. 3: oxygen limitation in aerobic fermentations plays a role in attainable cell density, nothing but air and alkali added during. Continuous fermentation: substrate is added continuously, total number of cells remain constant (cid:224) add and remove at the same time. Reduces production cost: use smaller bioreactors but same amount of product (cid:224) avoids down time between runs, smaller downstream processing equipment, keeps cells in more uniform state. Problem: difficult to maintain sterility, loss of plasmid fermentation, some cells spend most of their life in stationary phase (quiescent, lag phase (adapt, accelerations phase, logarithmic or exponential phase, deceleration phase, stationary phase, death phase desirable to monitor and control ph, temperature, dissolved molecular oxygen (hard to dissolve without breaking cells), stirring rate and foaming (causes inactivation of cells.
