BIOL432 Study Guide - Final Guide: Glycosylation
14 Apr 2015
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Document Summary
New ti plasmid based vectors are used with: selectable marker gene such as kanamycin resistance gene under control of plant transcriptional regulation signals, origin of dna replication that allows plasmid to replicate in e. coli and a. tumefaciens, right borer sequence of tdna region and sometimes the lb sequence, polylinker (mcs) to facilitate insertion of cloned gene, killer gene encoding toxin downstream from lb to prevent unwanted vector. Oleosins: fuse the foreign protein to plant oleosins (oil body protein found in the seeds, hydrophobic embedded with small oil droplets but the n terminus and c terminus are hydrophilic and exposed to the aqueous, purification of oil bodies is simple if a cleavable linker is included between the oleosin and the recombinant protein, seeds can be stored prior to purification without deterioration. Problematic carbohydrate residues: rnai constructs to interfere with plant enzymes that produce these problematic carbohydrate residues.
