KIN217 Study Guide - Final Guide: Differential Centrifugation, Immunoassay, Western Blot

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Chapter 1: biochemistry and the unity of life. Unity of biochemistry: organisms are uniform at the molecular level. Chapter 2: water, weak bonds and the generation of order out of chaos. Brownian motion: the movement of particles brown observed is due to the random fluctuation of energy content of the environment. Van der waals interaction: distribution of electronic charge around an atom changes with time and the charge distribution is not perfectly symmetric. Buffer: resists changes in the ph of a solution. Dipolar ions (zwitterions): free amino acids in solution at neutral ph. Primary structure: linear polymers formed by linking the alpha carboxyl group of one amino acid to the alpha amino group of another amino acid. Peptide bond (amide bond): the linkage joining amino acids in a protein. Disulfide bond: formed by the oxidation of a pair of cysteine residues. Phi angle: angle of rotation between the nitrogen atom and alpha carbon.

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