BIOL 112 Study Guide - Midterm Guide: Phosphodiester Bond, Chemical Bond, Covalent Bond

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BIOL 112 Full Course Notes

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Document Summary

Transcription is the first step in gene expression= production of functional gene product. Dna strand being transcribed runs from 3" to 5". s. Steps: separate dna strands, for rna-dna duplex, elongate transcript, release transcript, restore dna. In eukaryotes 6 proteins needed general transcription factors attract. Required: template dna, riboneucleoside triphosphates, rna polymerase, a large complex to occur in. Initatiating: regulatory transcription factors bind with dna sequence enhancer. General t factors recruit rna polymerase complex factors and transcription takes place. Translation is the 2nd step in which rna is used as code for aa. Dna is read in 3" to 5" direction whereas a new strand is synthesized in the 5" to 3" direction. Sugar linked to phosphate group on next nucleotide. Phosphate oxygen"s charged due to proton loss. Combination of sugar and base known as nucleoside. In eukaryotes dna packaging into 30nm chromatin fiber. + histone binds with sugar po4 backbone. Targeted region of dna to be replicated/amplified into many copies.

Related Questions

During transcription in eukaryotes, a type of RNA polymerase called RNA polymerase II moves along the template strand of the DNA in the 3'â5' direction. However, for any given gene, either strand of the double-stranded DNA may function as the template strand. Which of the following initially determines which DNA strand is the template strand, and therefore in which direction RNA polymerase II moves along the DNA?

A. the location of specific proteins (transcription factors) that bind to the DNA

B. the specific sequence of bases along the DNA strands

C. the location along the chromosome where the double-stranded DNA unwinds

D. the position of the geneâs promoter on the chromosome

E. which of the two strands of DNA carries the RNA primer

1. A region of a chromosome contains two strands of DNA, yet only one is used to transcribe a gene because

A. all genes are found on the same strand of DNA in a chromosome.

B.both strands are transcribed, but only one strand of DNA contains a reading frame.

C.RNA polymerase binds to the terminator, stopping transcription on one strand of DNA.

D.RNA polymerase binds to the promoter, which precedes the gene only on the template strand of DNA.

E.the start of transcription is random along the chromosome and doesn't always occur on a gene.

2. In eukaryotes an mRNA molecule can only be translated by one ribosome at a time.

True or False

3.The process by which cells use the information of RNA molecules to make proteins is transcription.

True or False

Which type of RNA is unique to eukaryotes?

		mRNA
		rRNA
		tRNA
		snRNA
		Its a trick, all cells have all four types of RNA.

1 points

QUESTION 2

The region of DNA to which RNA polymerase binds to initiate transcription is the:

		origin
		promoter
		enhancer
		none of the above are correct

1 points

QUESTION 3

Holoenzyme:

		is RNA polymerase core enzyme.
		is sigma factor.
		is core plus sigma factor.
		none of the above are correct.

1 points

QUESTION 4

Which of these is formed FIRST when RNA polymerase binds to a promoter?

		The closed promoter complex
		The open promoter complex
		A rho-independent terminator
		none of the above are correct.

1 points

QUESTION 5

A hairpin loop followed by a run of Us:

		is the sequence formed in the sense strand of a gene using a rho-independent terminator.
		is the sequence recognized by the rho protein.
		is the structure formed in RNA that causes rho-independent termination.
		is formed immediately after the open promoter complex is formed.

1 points

QUESTION 6

RNA polymerase:

		reads the template strand of the DNA in the 3' to 5' direction.
		synthesizes the RNA in the 5' to 3' direction.
		can begin transcription without a primer.
		all of the above are correct.

1 points

QUESTION 7

Which of the following statements is FALSE?

		Bacteria like E. coli have only one type of sigma factor.
		RNA polymerase has no proofreading activity.
		The promoter of a gene is upstream of the RNA-coding region.
		Bacterial transcriptional terminators are copied into RNA to cause termination.

1 points

QUESTION 8

If the template strand of a gene has the sequence 5' ATGCGAT 3', then:

		the RNA produced will have the sequence 5' AUGCGAU 3'.
		the RNA produced will have the sequence 5' UAGCGUA 3'.
		the RNA produced will have the sequence 5' AUCGCAU 3'.
		None of the above are correct.

1 points

QUESTION 9

The sense strand:

		is the lagging strand
		is also the template strand.
		always goes left to right.
		none of the above are correct.

1 points

QUESTION 10

Which of these is transcribed but not translated?

		mRNA
		tRNA
		rRNA
		all of the above
		tRNA and rRNA are transcribed but not translated.

please help i am a bit confused

BIOL 112 Study Guide - Midterm Guide: Phosphodiester Bond, Chemical Bond, Covalent Bond

BIOL 112 Full Course Notes

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