BIO207H5 Study Guide - Midterm Guide: Chromatin, Hemoglobin, 10 Nanometer
Document Summary
Crossing over that prevents lined genes from segregating together occurs during prophase i of meiosis. Interference = 1 coc: i < 0: negative interference: more dco than expected. Observed dco = coc (expected dco) x total progeny. Hemoglobin usually tetramers with two a-globin, two b- globin. Electrophoretic mobility: the characteristic rate of migration or final position of a protein. Individuals with different b-globin genotypes have different electrophoretic mobility (bs has lower mobility than ba) Densitometry: measure of how much light is blocked from passing through gel by protein in a band. Restriction fragment length polymorphism: inherited variability in number or length of fragments. Snp that alters sixth dna triplet of coding sequence causes scd: gag mrna glu -> gug mrna val. Southern blot: ba: two bands 1150, 200 bp, bs: one band: 1350bp. Bc allele in west africa; glu into lys in 6th pos. Be se asia; severe anemia; glu to lys in 26th pos.
