BIO314H5 Study Guide - Final Guide: Nanopore, Synthetic Membrane, Expression Vector
Document Summary
Sanger- know how to read and what the template strand would have been. High through put- 3 next gen sequencing approaches that we are supposed to know illumine, smrt, nanopore. How are they converted to something that can be registered electronically. In nanopore we have a very electrically resistant artificial membrane with proteins embedded in it and the nuc pass thru and the electrical field- electrical resistance which we can register. Changing color as bases are added- so enable a visual output by amplifying. And we need to know sanger termination methods ddntp. Electrophoresis- depending on what we want to get out. Pcr ta cloning- what is the same about the at vector as conventional cloning vector pfu would chew them off in the end. What is the feature of the plasmid vector. Florescent prot tree- need the coding sequence- say gfp- and then we got our gene of interest.
