BIO315H5 Quiz: Tutorial Test 1 Notes These study notes cover all the material included on tutorial test 1. They are concise and to the point to help reduce study time while still preparing effectively. Material is presented in the order in which they were introduced in

1) You would like to determine the lacZ expression in thepresence and absence of lactose containing media in E.coli. Which of the following experimental techniques willallow you to do so?

Multiple choices are possible

In 200 words or less, explain what phallotoxins are, and how their derivatives are now being used in cell biology research. Specifically, how they are used in conjunction with fluorescence microscopy. (5 points)

26.The best microscopy technique for observing different subcellular structures on the surface of bacteria is

(A) SEM (B) TEM

(C) phase contrast microscopy (D) interference microscopy

(E) fluorescence microscopy

27.Which type of microscopy exploits differences in the refractive index of the specimen, where light is retarded by one-quarter of a wavelength when it passes through a bacterial cell?

(A) SEM (B) TEM

(C) phase contrast microscopy (D) bright-field microscopy

(E) fluorescence microscopy

28.Which type of microscopy should be used to examine bacterial cells that have been Gram stained?

(A) TEM (B) fluorescence microscopy

(C) phase contrast microscopy (D) interference microscopy

(E) bright-field microscopy

29.Which of the following is most abundant in a prokaryotic cell (in dry weight)?

(A) RNA (B) DNA

(C) Polysaccharide (D) Protein

(E) Lipid

30.Which of the following techniques uses the Svedberg unit (S)?

(A) electron microscopy (B) ultracentrifugation

(C) X-ray crystallography (D) genetic analysis

(E) sonication

31.Which of the following is NOT a function performed by the cell membrane or its components?

(A) production of proteins (B) detection of environmental signals

(C) transport of molecules into and out of the cell (D) separation of charge across the membrane

(E) B and D

32.Which of the following statement is true?

(A) Lipopolysaccharides (LPS) are found in the cell wall of Gram-negative bacteria.

(B) Lipopolysaccharides (LPS) are found in the cell wall of Gram-positive bacteria.

(C) Teichoic acids are found in the cell wall of Gram-negative bacteria.

(D) Teichoic acids are found in the cell wall of Gram-positive bacteria.

(E) Cholesterol is found in the cell wall of Gram-negative bacteria.

33.Which of the following is found in Archaeabut not in Bacteria?

(A) Hopanoids (B) Terpenoids

(C) Cholesterol (D) S-layer

(E) Peptidoglycan

34.The glycan chain of archaeal cell wall is made of __________ and __________, while the glycan chain of bacterial cell wall is made of __________ and __________.

(A) peptidoglycan and murein, pseudopeptidoglycan and murein

(B) N-acetylglucosamine and N-acetyltalosaminuronic acid,

N-acetylmuramic acid and N-acetyltalosaminuronic acid

(C) N-acetylglucosamine and N-acetyltalosaminuronic acid,

N-acetylglucosamine and N-acetylmuramic acid

(D) N-acetylglucosamine and N-acetylmuramic acid,

N-acetylglucosamine and N-acetyltalosaminuronic acid

(E) N-acetylmuramic acid and N-acetyltalosaminuronic acid,

N-acetylglucosamine and N-acetyltalosaminuronic acid

35.The sacculus of bacteria is composed primarily of

(A) phospholipids (B) peptidoglycans

(C) carbohydrates (D) amino acids

(E) nucleic acids

36.Lysozyme attacks the following linkage in the bacterial cell wall:

(A) β(1,4)-glycosidic bonds

(B) β(1,3)-glycosidic bonds

(C) Glycine peptide interbridges

(D) Peptide cross-links between glycan chains

(E) Peptide bonds between L-alanine and D-glutamic acid