BIO315H5 Study Guide - Midterm Guide: Signal Peptide, Ubiquitin, Lysine
Document Summary
E1 - takes carboxy terminal of ubiquitin and adds it to cysteine sidechain on e1. E1(activating) interacts with e2 (conjugating), then transfers to e3. E3 has no enzymatic activity, takes e2 to have an activity. This picture only shows 2 (in grey and salmon). Nucleus is topologically the same as the cytosol. We don"t need to penetrate any membrane to move from one lumen to another. If we start of n-terminal is pointing to the cytosol, if we move to another space, this n-terminal is still pointing to the cytosol. Be able to explain in detail nuclear import and nuclear export. Nuclear import - fg repeats, ran (monomeric g protein) = high ran-gdp in the cytosol, and high ran-gtp in the nucleus. nuclear import receptors bind to ran-gtp. Describe how proteins are targeted to the er. Er signal sequence, srp (brings nascent polypeptide to the receptor), srp receptor.
