BIO370Y5 Quiz: Cheat sheet - after test 1
Document Summary
Galactosidase (z gene) splits lactose into glucose and galactose. Lactose becomes allolactose which is the actual inducer. Permease (y gene) an inner membrane protein which increases lactose uptake. I- = consituive mutant (always make all three proteins even in the absence of inducer). Is prevented inducion of -gal even when lactose or iptg present. Cap binds to camp this complex binds to lac promoter dna bends and acivate transcripion. D(epimerase), enzymes were produced only when both arac(posiive-acing regulator) protein and arabinose were present 1946- lederberg and tatum - demonstraion of geneic recombinaion using triple auxotrophs. Specialized transducion: only certain loci into the new host cells this occurs only in temperate phages and is dependent on mistakes being made in the excision of the prophage. The resuling phage genome contains porions of the bacterial chromosome (5 to 10% of the bacterial dna) that were next to the integraion site. In the lyic cycle, incorporated host genes are replicated along with phage.
