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BIOC 299 (5)
Midterm

BIOC 299 Midterm: UVic Bioc 299 Midterm 2 Notes
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Department
Biochemistry
Course
BIOC 299
Professor
Briant Douglas
Semester
Winter

Description
Chapter 16- Glycolysis • Enzymatic Steps o 10 successive reactions that occur in the cytoplasm + o Glycolysis net +eaction: glucose + 2 ADP + 2 NAD + 2 P  2 piruvate + 2 ATP + 2 NADH + 2 H + 2 H O 2 o Three stages of glycolysis: ▪ 1. Trap and destabilize glucose • Kinases are enzymes that catalyze the transfer of a phosphoryl group from ATP to an acceptor 2+ • Hexokinase, as well as other kinases, require Mg for activity • Two lobes of hexokinase move together when glucose is bound (induced fit) • Substrate-induced cleft closing is a general feature of kinases • Next step is isomerization of glucose 6-phosphate to fructose 6-phosphate • The isomerization of glucose 6-phosphate to fructose 6-phosphate is a conversion of an aldose to a ketose, reaction is catalyzed by phosphoglucose isomerase. • Isomerization important because later only 3 carbon molecules are metabolized • Fructose 6-phosphate is phosphorylated by ATP to fructose 1,6- biphosphate • Phosphorylation is one of the major regulatory points in glycolysis, big drop in free energy and energetically favorable • This irreversible reaction is catalyzed by phosphofructokinase (PFK) • Phosphofructokinase is an allosteric enzyme that is the key regulatory enzyme for glycolysis • ▪ 2. 6 to 3 C molecule conversion • The fructose 1,6-bisphosphate is split into two triose phosphates, glyceraldehyde 3-phosphate (GAP), ad dihydrozyacetone phosphate (DHAP) • This is catalyzed by aldolase • The triose phosphate isomerase catalyzes the isomerization of three- carbon phosphorylated suga
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