BIOL 465 Study Guide - Final Guide: Exome Sequencing, Mhc Class I, Sanger Sequencing

• School: University of Victoria
• Department: Biology
• Course: BIOL 465
• Professor: Walter Patrick

Document Summary

Exome analysis t cell therapy (excluding sanger sequencing & ngs) Exome sequencing technique to sequence only protein-encoding genes (exons) Typically, only coding genes responsible for phenotypes in a cell. Dsdna is fragmented, adapter sequences added to ends, hybridize on flow cells for target enrichment. Advantages: exons only accounts for 1% of genome. ~ 180k exons = 30 million bps: misses, cost effective, quick, catches/includes, deletions, variants, gene-rearrangements, introns (w/ start txn sites, micrornas (that regulate txn, point mutations, copy number variations. Macrophages = responsible for antigen processing & presentation: epitopes displayed on mhc receptors that present it to t-cell on its tcr. Mhc-ii cd4+ (antigen: activated cd4+ t cell (by mhc class ii) will release, cytokines activating other immune cells (nk cells tumor/infected cell, ifn- ( cd8+ t cell) Cytokine: activated cd8+ t cell (by mhc class i) will release, cytokines activating other immune cells (nk cells, other macrophages, perforin & granzyme to kill tumor/infected cell.