Biology 2581B Study Guide - Quiz Guide: Dna-Binding Domain, Atp Hydrolysis, Cooperative Binding
3 Jun 2020
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Memory of the system: importance of positive feedback in gene expression induced. Take fully induced cell, reduce lactose concentration of surrounding medium, remains: once permease expressed positive feedback since only then may more lactose. Once polymerase open conformation pretty irreversible will happily transcribe. Not fully induced cell- variation in operon activity enter and decrease repression. Cap-camp: co-operative binding of above camp increases affinity of cap co-operative binding with rna polymerase and promoter - Helix turn helix dna binding motif v common in e. coli gene regulation equivalent of. Regulate many genes, not just lac: no atp hydrolysis / conformational change required eukaryotic homeodomain significant frequency of binding by self, otherwise constitutive or never active. Not detected cap polymerase interaction in absence of dna. Cap constantly made, activity modulated by camp ac activity altered by glucose transport. How we know about cap"s mode of action:
