PLANTBI C112 Midterm: 10 Counting Bacteria 2

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Counts number of cells in a sample capable of growing into a colony on solid medium. In theory - 1 colony is derived from one cell, can only count living cells. If counting cells from env sample, medium may be unsuitable for some species. Cells may grow but not form colonies. Pipette cells into hemocytometer and count cells within grids. Polycarbonate membrane lters: count cells on the lter from 10. Elds of view, average, and multiply by total number of elds. Optical density/turbidity : proportional to the number of cells present. Visualizing proteins within cells to understand cellular organization: An antibody that recognizes a speci c protein is covalently attached to a. Fluors are molecules that absorb light at one wavelength (shorter = higher: and emit light at another wavelength (longer = lower e) Fixed, permeabilized cells are treated with the antibody and observed under a microscope.

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