CAS BI 410- Midterm Exam Guide - Comprehensive Notes for the exam ( 39 pages long!)
13 Oct 2017
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Fig 1 = characterization membrane moves microtubules are stationary (don"t show an active role in protrusion) Actin is being polymerized and becomes dense, forming a network = too many stress fibers. Use dominant negative rac1 (dysfunctional and inhibits all other. Fig 6: kymograph along one white line white=retraction black=protrusion. Mt dynamics required to reduce adhesions and to allow traction for migration (gof) Fig 8:noc= do migrate but extend like a snakes: fail to retract therefore: mt dynamics required for tail retraction. Migration is stimulated (with scf), and in cells treated with nocodazole, the cells do migrate, but stretch out like snakes compared to the controls, indicating a problem with retraction. The conclusion is that microtubule dynamics are required for retraction. 8, but in this example, the cell"s tail has torn off. This suggested that the basis of the retraction issue with nocodazole (n) (and taxol, t) is too much adhesion.
