MICR-4150 Study Guide - Midterm Guide: Bioluminescence, Quorum Sensing, Chemotaxis
30 Dec 2019
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Mcps = methyl-accepting chemotaxis proteins (membrane receptor kinases) Run = in low concentration of attractant. Tumble = in a high concentration of attractant. Quorum sensing: cell to cell communication mediated by small molecules. Cells density + intercellular communication to regulate transcription. Autoinducer here is small enough to diffuse out. Luxr - transcription activator (active when bound to ahl) Help identify self-species vs self-genus vs bacteria. Activator for bioluminescence genes, inhibits secretion system iii. Low cell density vs high cell density. Not enough aa: empty trna tells rela to do two things. Make less trna and rrna => not enough aa. His auxotrophs: grow only on media containing histidine means it doesn"t make it"s own his (auxotrophs - cannot synthesize necessary compound for growth) Antibiotic resistance: grow on antibiotic plates and see who survives. Motility/sporulation: depend on the understanding of physiology. Silent mutation: alters codon but makes the same amino acid. Protein will become completely, partially or conditionally nonfunctional.
