BIOMI 2900- Final Exam Guide - Comprehensive Notes for the exam ( 45 pages long!)

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Biomi2900 lecture 1 history of microbiology: microbiology before the microscope, early microbiologists cannot see samples in petri-dish, examples of microbe action, contagious diseases, tried to come up with scientific reasons for cause (difficult without instruments, malaria. Incredibly small: 100 of them length of a grain of coarse sand, observations on animalcules in the mouth (dental plaque + water, microscopy, louis pasteur (1822-1895, french chemist, studied rotation of polarized light by organic compounds. Biomi2900 lecture 2 woese, pace and the big tree, the small world of microbes. Lecture 1 history of microbiology continued : robert koch (1843-1910, developed koch"s postulates, central points. Isolated rhizobium from root nodules of n2-fixing legumes. G, and compared words >_ 6 nt long: now a simple pcr method used to amplify dna encoding rrna, 16s rrna structure, 2d structure shows external base-pairing, an unexpected result with methanogens. Low g + c gram(+) includes: pathogens like streptococcus and staphylococcus, endospore formers like bacillus (aerobes) and clostridium, actinobacteria.

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