BIOL 202 Quiz: Chapter 3 Study Guide
8 Feb 2017
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Chapter 3: concepts and tools for studying microorganisms. Acid-fast technique: staining technique for mycobacterium: bacterial cells are stained red with carbol fuschin. Acidic dye: anionic dye that is repelled by the negatively charged bacterial cell membrane: the field is colored, rather than the bacteria, cells are observed in their natural conditions. Antiserum: the collection of antibodies that are generated by the immune system as a result of microorganism infection. Archaea: domain and kingdom of prokaryotes: archaea do not have peptidoglycan on cell wall. Basic dye: cationic dye attracted to negative cell wall: cells are stained while field remains the same, size and morphology. Biofilm: complex communities of bacteria that communicate and interact to thrive in environment. Bioremediation: the industrial repurposing of bacteria and biofilm to remove waste, etc. Differential staining procedure: stain with purple dye; add iodine solution; add alcohol and assess whether or not color is lost; determine gram positive/negative; size and morphology.
