BIOLOGY 557L Study Guide - Midterm Guide: Microbiology, Mycobacterium, Chemotherapy
Document Summary
Artwork by stacie bumgarner, whitehead institute for biomedical sciences, cambridge, ma. Address the issue of un-cultivability of microorganisms in the lab using traditional agar or liquid nutrient medium-based approach, i. e. the great plate count anomaly (colwell & The dependency of microorganism on their rather complex natural environment (including other microorganisms, nutrients, and other growth factors found in the original habitat) for growth (kaeberlein, lewis, & epstein, 2002). Discrepancy of microbial diversity predicted by rrna analysis and cultured microorganisms (barer & harwood, 1999; rapp & giovannoni, 2003) Cells grown by ichip have been shown to be different from those in petri-dishes (sherpa reese, Ability to sub-culture isolated bacteria in standard method post-ichip remains challenging despite domestication to traditional in vitro culturing techniques (sherpa reese, & aliabadi, Structure (sherpa reese, & aliabadi, 2015; stanley et al. , 2016): Contains a set of 3 flat hydrophobic plastic polyoxymethylene (pom) thermoplastic plates with holes arranged in two 192-hole array in diffusion chambers per each plate.
