BSC 4930 Study Guide - Midterm Guide: Fate Mapping, Forward Genetics, Genetic Screen
Document Summary
Early theories of development: preformation: miniature organism already formed in the egg/sperm, epigenesis: processes that divide and differentiate cells; increase in organization. Fate mapping: which tissues/organs are derived from what parts of the embryo: labeling the embryo and looking for the label within the developed organism. Forward genetics: unbiased genetic screen that looks for mutations affecting a process: mutation gene. Backwards: starting with a gene and trying to figure out what it does: gene mutation. Zygotic effect vs maternal effect (genotype of the mother is critical) Microscopy: confocal lets you look at a specific focal plane. Fluorescence: tags different things to see how/where certain genes express. Tem: within cells; sem on the surface of the cells. Models: good characteristics: large/easily obtainable, relevant, genetics well understood, big 6: match common and scientific and advantages. Control of gene expression- different levels: regulatory proteins-transcription factors- interact directly with dna. Specific: tissue-specific, spatial and temporal regulation; where and.
