PCB 3063 Study Guide - Comprehensive Final Exam Guide - Dna, Protein, Rna

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Published on 20 Nov 2018
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PCB 3063
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De novo
- starts from the beginning = doesn't require a primer
- ex: RNA synthesis can be "de novo"; DNA synthesis can't be
Taylor, Woods, Hughes experiment
- pulse chase style on beans
- used ³H thymidine
Procedure:
- grow beans in radioactive ³H thymidine
- switch to cold (non radioactive) thymidine
- stop cells at metaphase
- squash cells and dip them in photographic emulsion
Findings:
- inactivated X replicated last
- euchromatin replicates early
- heterochromatin replicates late
- homologous recombination
Autoradiography
image of radioactive particles as they degrade
Pulse-Chase experiment
pulse = cells are exposed to a labeled (ex: radioactive) compound
chase = cells are in normal version of that compound
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Endocycle
replication of DNA without mitosis or meiosis
OriC
(in prokaryotic replication)
- DNA sequence 230bp
- consensus sequence
- 9mers = 9 base con. seq.
- 13mers = 13 base con. seq.
consensus sequence
alignment of bases or amino acids to identify conserved sequences
dnaA
(in prokaryotic replication)
- binds oriC
- changes conformation of double helix
- opens bubble at AT rich region of oriC
- recruits dnaB, dnaC
dnaB
(in prokaryotic replication)
- aka helicase
- hexamer(6 subunits)
dnaC
(in prokaryotic replication)
- chaperone
- aids in the binding of dnaB
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