PCB 3063 Study Guide - Comprehensive Final Exam Guide - Dna, Protein, Rna
Document Summary
Starts from the beginning = doesn"t require a primer. Ex: rna synthesis can be "de novo"; dna synthesis can"t be. Squash cells and dip them in photographic emulsion. Autoradiography image of radioactive particles as they degrade. Pulse-chase experiment pulse = cells are exposed to a labeled (ex: radioactive) compound chase = cells are in normal version of that compound. Endocycle replication of dna without mitosis or meiosis. 13mers = 13 base con. seq. consensus sequence alignment of bases or amino acids to identify conserved sequences dnaa (in prokaryotic replication) Opens bubble at at rich region of oric. Recruits dnab, dnac dnab (in prokaryotic replication) Aids in the binding of dnab dnag (in prokaryotic replication) No phosphodiester bond between 3"oh and 5"po4 on adjacent okazaki fragments. Fixed by ligase and atp topoisomerase ii (in prokaryotic replication) aka gyrase. Breaks phosphodiester bonds of both strands to pass one strand through and reduce coiling tus.
