PCB 2061 Study Guide - Quiz Guide: Nucleic Acid Thermodynamics, Enzyme, The Strongest
Document Summary
Domain 1. 1 is blocking the region where dna is located when the promoter is melted. Domain 1. 1 prevents sigma from binding promoter without first binding the rnap core. If domain 1. 1 is deleted, sigma will bind promoter sequence. Domain 1. 1 is blocking the region where dna is located when the promoter is melted. w/ Domain 1. 1 prevents sigma from binding promoter without first binding the rnap core. w/ Domain 1. 1 helps melt the promoter sequence and create an open promoter complex. Through contacts of sigma subdomains 2. 4/2. 3 and 4. 2 and cis-acting promoter elements. Grea and greb; reposition mg2+ ions in the active site, which makes rnap cleave trailing end off nascent rna to align it correctly in the catalytic site. Transition from closed to open promoter complex happens readily at higher temperatures (dna melting); open promoter complex is most stable at 37 deg and the least stable at 15 deg.
