BIOE-110 Study Guide - Midterm Guide: Size-Exclusion Chromatography, Isoelectric Point, Dehydration Reaction

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Hydrogen bonding: + hydrogen atom attracted to - nitrogen, oxygen, or fluorine, can. Ionic bonds: formed when electrons are transferred from on atom to another atom. Covalent bonds: sharing of electrons between atoms; can be polar or nonpolar. Van der waals: weak, non-covalent interactions be polar or nonpolar, relatively weak. Functional groups: convey properties of biological molecules, can participate in chemical reactions. Amino acid de-protonation: pk1 refers to -carboxyl group; pk2 refers to -amino group. Coo- pi = isoelectric point; charge balances out with neutral pk1 pk2. Amino acid bonding: c-terminus of one amino acid binds to n-terminus of another amino acid via a dehydration reaction, forming a peptide bond. Nucleic acid bonding: adenine and thymine are linked by two hydrogen bonds; guanine and. Plasmid map: a plasmid is a circular piece of dna often found in bacteria; can replicate independently of other dna; contains restriction sites as well as resistance genes; contains origin or replication.

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