BIOL 1002 : Test 4
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1. How can scientists use the CRISPR/Cas9 system used to mutate a gene? (Choose ALL correct answers)
[] | Cas9 and the RNA it binds to can be put into cells by scientists in order to induce breaks at specific DNA sequences |
[] | once the DNA is cut, repair mechanisms of the cell can make changes to the DNA at that specific site |
[] | Cas9 binds with the transcribed mRNA from the gene and interferes with translation so that the protein is never produced |
[] | Cas9 can bind to the promoter of the gene to change the frequency of gene transcription |
2. Which of the following are features of the CRISPR/Cas9 system? (Choose ALL correct answers)
[] | Cas9 uses RNA to find matching sequences of DNA |
[] | Cas9 makes double-stranded breaks in specific sequences of DNA |
[] | Cas9 makes random double-stranded breaks in DNA |
[] | Cas9 makes breaks in RNA that are targeted and specific |