BIOL 1208 : Biology 1208 FINAL
Document Summary
Pipets: long graduated tubes used to accurately measure and transfer small volumes (10 ml or less) Small volume pipets: transfer, pasteur, micro (aka mechanical, specifies volume through three digit window, used for less than or equal to one ml (1000 microliters, l, pxxx stands for the maximum volume. **in transferring solutions, use pipets that can hold the desired volume in order to provide the best accuracy. **green is for 10ml pipet, blue is for 1ml pipet. Spectrophotometer: instrument designed to detect the amount of radiant energy absorbed or transmitted by colored molecules. How it works: diffraction grating splits light into its component wavelengths (prism) **always wipe the cuvette before putting it in the spec because dirt, oil, and grim on fingertips prevents light from effectively going through the glass, giving erroneous absorbance readings. Percent transmittance (0-100%: used to zero and blank. **the relationship between transmittance and absorbance is inversely proportional (for example, a.
