BIOL 2051 Study Guide - Midterm Guide: Ethylene Oxide, Asepsis, October 8
Document Summary
Experiment 1- media preparation and sterilization: solid medium contains agar (from red algae) Deep anaerobic growth: complex medium composed of digests of chemically undefined substances such as yeast and meat extracts, defined medium medium whose precise chemical composition is known. Experiment 2- culturing microorganisms from the environment: minority of microorganisms are pathogenic- disease causing agent, nosocomial means hospital-acquired. Purpose: this experiment will demonstrate the ubiquitous nature of microorganisms. Materials needed: 1 trypticase soy (ts) broth, 2 ts agar plates, 2 sterile swabs. Experiment 3- hand washing: resident (normal) microbiota/flora occur naturally on or in body, transient flora temporary on body, can"t grow on skin because skin is too dry and acidic, nosocomial infection hospital-acquired infection. Infectious dose number of microorganisms required to establish an infection. Purpose: this experiment will evaluate effectiveness of different soaps & hand washing times. 10. colony - a group of genetically identical bacteria, arising from a single cell on an agar plate.
