BIOL 2153 : Exam 2
Document Summary
Overview of transcription and translation: central dogma. Inside the transcription bubble about 9 rna nucleotides are base paired in a temporary rna-dna hybrid: the new rna strand exists the enzyme as a single strand, polymerase has 2 proofreading activities. It will back up and remove an incorrect nucleotide and replace it with the correct one when going forward again. It moves back one or more nucleotides and cleaves the rna at that position before resuming rna synthesis going forward: termination, terminator, specifies where transcription stops, terminator sequences. Inr (initiator) defined as +1: tata box (tata element) located. In eukaryotes tata box -30 about -30: 5"- tataaaa -3", also called goldberg-hogness, promoter-proximal elements box, located upstream from the tata box, about. The dna containing the enhancer is brought close to the promoter. Dna to which the transcription machinery is bound, stimulating transcription to the maximum level for the particular gene.
