BIOL 2153 : Genetics Test 3 Corrections
Genetics Test 3 corrections:
• A nucleoside with a phosphate ester linked to the sugar is a nucleotide
• Based on Griffith’s results, what would you expect if you injected both heat-
killed RII and heat-killed SIII…The mouse lives (because both of them are
killed so there is nothing to cause death)
• X-ray data showed that the DNA helix undergoes one complete turn every 34
angstroms
• B-form DNA spirals to the right and is the major form of naturally occurring
DNA molecules
• Which of the following is not involved in ensuring the accuracy of cell’s
genetic information? (redundancy, repair enzymes, precision of replication
machinery, DNA polymerase proofreading mechanism, restriction
endonucleases)
• Assume that plasmid (circular) is 3200 base pairs in length and has
restriction sites at the following locations: 700, 1400, 2600. Give the
expected sizes of the restriction fragments… forgot answer
• False; the more condensed a part of a chromosome is, the more likely it is
that the genes in the region will be active
• Bacteria protect their own DNA from restriction enzyme damage by adding
methyl groups to certain nucleotides
• RFLPs arise due to base changes that add or delete restriction enzyme
recognition sites & addition or deletion of bases between restriction sites
• If the recognition sequence of the enzyme Few I is 5’-T^CGA-3’ about … see
paper…
• A mutation is known in which a single nucleotide is deleted at the asterisk
position indication, the deletion cause a premature stop codon to be
introduced, resulting in a defective non functional human grown factor
o If DNA is extracted how many different sized frags would be
detected… 3 (the mutation will not cut)
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