GLG 111 Study Guide - Congo Red, Gram-Positive Bacteria, Microscope Slide
Document Summary
Know the basic aseptic technique that you practice in the lab. Terms to know: brightfield microscope, working distance, total magnification, magnification, resolution, parfocal, parcenter. Know what should happen on the microscope to get the best resolution possible. Know about the wet mount and hanging drop techniques. Know what m. luteus and m. roseus look like on a slant. Know some possible sources of contamination when transferring bacteria. Terms to know: heat fix, direct stain, negative stain, agar, slants, pour plates, simple stain. Know when it is and is not appropriate to heat fix a smear. Know the stains methylene blue, crystal violet, safranin, congo red, and nigrosin . Terms to know: differential staining, the gram stain. Know the reagents used in the gram stain and their function. What does the gram stain actually stain? (bacteria or background) Difference between gram positive and gram negative bacteria. The organisms used in the lab and their gram stain reaction.
