VB SC 050S Study Guide - Quiz Guide: Immunodiffusion, Precipitin, Direct Examination
25 Aug 2016
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Document Summary
May be performed on live animals or at post-mortem examination. Techniques for detection of the infectious disease agents: techniques to demonstrate presence of infectious agent, direct examination of tissues or tissue smear, light microscopy. Proper sampling technique: growth requirements of infectious agent. Pcr: techniques to demonstrate host reaction to infectious agents, detect level of antibodies to specific organism, antibody titer, precipitin reaction, agid- agar gel immunodiffusion, elisa- enzyme linked immunosorbent assay. Bacteria/fungi/protozoa: genetic information is dna (double stranded, microscopic, produce energy, produce a vast array of macromolecules, self replicating. Virus: dna or rna (can be single stranded, sub-microscopic, unable to produce energy, limited macromolecule synthesis, do not self-replicate. Discovery of viruses : occurred in 1898, loeffler & frosch working on fmd, took a contaminated sample, introduced it to animal & produced the disease, took contaminated sample, passed it through a very fine filter, produced the disease.
