CHE 276 Study Guide - Quiz Guide: Boiling Point, Elution, Sodium Hydroxide
Document Summary
A sample for thin layer chromatography containing two compounds (say, two dye molecules) was contaminated by a student with two other compounds. The next student came and run the tlc of the contaminated sample. Yes they would still get accurate rf values, except there would be more spots on the. Tlc plate causing someone to get multiple rf values. The tlc of a compound is run twice, once in a polar solvent, the other in a non- polar solvent. The polar solvent has higher rf then the nonpolar solvent. When a mixture of two compounds are eluded through a column to be separated, but the compounds never come out after a long time. What may likely be the solution to this problem? a. When a mixture of two compounds is eluded through a column to be separated, both compounds come out rather quickly as a mixture without any separation.
