BSC 450 Study Guide - Midterm Guide: Nitrocellulose, Carl Bosch, Cell Nucleus
13 Oct 2017
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The following sections of chapter 3 and 4 will be included in the exam. Please read these sections: chapter 3. 3, working with proteins. Page 89-94: chapter 4, box 4-5: methods for determining the 3d structure of a protein. Study guide for protein technologies: briefly explain how each of following chromatographies separates protein mixtures, ion exchange, - exploits differences in the sign and magnitude of the net electric charge of proteins at a given ph. The column matrix is a synthetic polymer containing bound charged groups; those with bound anionic groups are called cation exchangers, and those with bound cationic groups are called anion exchangers. With cation exchangers, proteins with a more negative charge move faster and elute earlier: size exclusion, - super large protein molecules flow and wash out first, short path length. Medium size molecules elute later, longer path length. Super long path length, enter all agarose beads: affinity binding ligand to protein interactions are noncovalent.
